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Drug target miRNA : methods and protocols / edited by Marco F. Schmidt.

Contributor(s): Schmidt, Marco F [editor. ] | Springer Science+Business Media [copyright holder.].
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1517,; Springer protocols (Series): Publisher: New York : Humana Press, [2016]Copyright date: ©2017Description: xi, 319 pages : illustrations (some color) ; 26 cm.Content type: text Media type: unmediated Carrier type: volumeISBN: 9781493965618 (hbk); 1493965611 (hbk).Subject(s): MicroRNA -- Laboratory manualsDDC classification: 572.8 Online resources: Table of contents
Contents:
miRNA targeting drugs : the next blockbusters / Marco F. Schmidt -- Functional analysis of miRNAs using the DIANA tools online suite / Ioannis S. Vlachos and Artemis G. Hatzigeorgiou -- Non-nucleotide modification of anti-miRNA oligonucleotides / Kim A. Lennox, Christopher A. Vakulskas, and Mark A. Behlke -- Quantification of oligonucleotide association with miRNA-argonaute complexes in vitro / Dimitrios G. Zisoulis -- Determination of anti-miR association with miRNA/argonaute complexes in vivo / Dimitrios G. Zisoulis -- Competitive argonaute-based RNA immunoprecipitation for investigation of transcriptomic response to anti-miR / John R. Androsavich -- Assessing anti-miR pharmacology with miRNA polysome shift assay / John R. Androsavich -- Evaluating synergistic effects of miR-34a mimics in combination with other therapeutic agents in cultured non-small cell lung cancer cells / Jane Zhao and Andreas G. Bader -- Assessing the off-target effects of miRNA inhibitors on innate immune toll-like receptors / Geneviève Pépin, Jonathan Ferrand, and Michael P. Gantier -- Design of multimodal small molecules targeting miRNAs biogenesis : synthesis and in vitro evaluation / Duc D. Vo and Maria Duca -- Machine learning approaches toward building predictive models for small molecule modulators of miRNA and its utility in virtual screening of molecular databases / Vinita Periwal and Vinod Scaria -- Identification of small molecule modulators of microRNA by library screening / Zhangang Xiao and Yangchao Chen -- Rapid generation of miRNA inhibitor leads by bioinformatics and efficient high-throughput screening methods / Christopher L. Haga, Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney, and Donald G. Phinney -- Peptide-based inhibition of miRNA-guided gene silencing / Johannes Danner, Balagopal Pai, Ludwig Wankerl, and Gunter Meister -- Small molecules targeting the miRNA-binding domain of argonaute 2 : from computer-aided molecular design to RNA immunoprecipitation / Teresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti, and Francesco Fazi -- Surface plasmon resonance : a useful strategy for the identification of small molecule argonaute 2 protein binders / Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellissimo, Francesco Fazi, and Gianni Colotti -- Antagonists of the miRNA-argonaute 2 protein complex : anti-miR-AGOs / Marco F. Schmidt, Oliver Korb, and Chris Abell -- Elucidating mechanisms of molecular recognition between human argonaute and miRNA using computational approaches / Hanlun Jiang, Lizhe Zhu, Amélie Héliou, Xin Gao, Julie Bernauer, and Xuhui Huang -- Kinetic analysis of target RNA binding and slicing by human argonaute 2 protein / Sarah Willkomm and Tobias Restle -- Site-specific fluorescent labeling of argonaute for FRET-based bio-assays / Sarah Willkomm, Adrian Zander, and Dina Grohmann -- Single-molecule fluorescence energy transfer assays for the characterization of reaction pathways of miRNA-argonaute complex / Myung Hyun Jo and Sungchul Hohng.
List(s) this item appears in: Pharmacy 2017-2018
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572.8 S.M. D 2017 (Browse shelf) Available 00017956

"Published In: United States, 07 December 2016"--Distributor's website.

Includes bibliographical references and index.

miRNA targeting drugs : the next blockbusters / Marco F. Schmidt -- Functional analysis of miRNAs using the DIANA tools online suite / Ioannis S. Vlachos and Artemis G. Hatzigeorgiou -- Non-nucleotide modification of anti-miRNA oligonucleotides / Kim A. Lennox, Christopher A. Vakulskas, and Mark A. Behlke -- Quantification of oligonucleotide association with miRNA-argonaute complexes in vitro / Dimitrios G. Zisoulis -- Determination of anti-miR association with miRNA/argonaute complexes in vivo / Dimitrios G. Zisoulis -- Competitive argonaute-based RNA immunoprecipitation for investigation of transcriptomic response to anti-miR / John R. Androsavich -- Assessing anti-miR pharmacology with miRNA polysome shift assay / John R. Androsavich -- Evaluating synergistic effects of miR-34a mimics in combination with other therapeutic agents in cultured non-small cell lung cancer cells / Jane Zhao and Andreas G. Bader -- Assessing the off-target effects of miRNA inhibitors on innate immune toll-like receptors / Geneviève Pépin, Jonathan Ferrand, and Michael P. Gantier -- Design of multimodal small molecules targeting miRNAs biogenesis : synthesis and in vitro evaluation / Duc D. Vo and Maria Duca -- Machine learning approaches toward building predictive models for small molecule modulators of miRNA and its utility in virtual screening of molecular databases / Vinita Periwal and Vinod Scaria -- Identification of small molecule modulators of microRNA by library screening / Zhangang Xiao and Yangchao Chen -- Rapid generation of miRNA inhibitor leads by bioinformatics and efficient high-throughput screening methods / Christopher L. Haga, Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney, and Donald G. Phinney -- Peptide-based inhibition of miRNA-guided gene silencing / Johannes Danner, Balagopal Pai, Ludwig Wankerl, and Gunter Meister -- Small molecules targeting the miRNA-binding domain of argonaute 2 : from computer-aided molecular design to RNA immunoprecipitation / Teresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti, and Francesco Fazi -- Surface plasmon resonance : a useful strategy for the identification of small molecule argonaute 2 protein binders / Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellissimo, Francesco Fazi, and Gianni Colotti -- Antagonists of the miRNA-argonaute 2 protein complex : anti-miR-AGOs / Marco F. Schmidt, Oliver Korb, and Chris Abell -- Elucidating mechanisms of molecular recognition between human argonaute and miRNA using computational approaches / Hanlun Jiang, Lizhe Zhu, Amélie Héliou, Xin Gao, Julie Bernauer, and Xuhui Huang -- Kinetic analysis of target RNA binding and slicing by human argonaute 2 protein / Sarah Willkomm and Tobias Restle -- Site-specific fluorescent labeling of argonaute for FRET-based bio-assays / Sarah Willkomm, Adrian Zander, and Dina Grohmann -- Single-molecule fluorescence energy transfer assays for the characterization of reaction pathways of miRNA-argonaute complex / Myung Hyun Jo and Sungchul Hohng.

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